Published customer image:
Hamster anti Mouse CD11c antibody, clone N418 (MCA1369GA) used for the detection of CD11c expressing cells in mouse spleen by immunofluorescence.
Image caption:
Mannose receptor positive and negative APC subsets identified by immunofluorescence microscopy. Tissues were examined by immunofluorescence microscopy for MR expression and other APC phenotypic markers in dual-labeled specimens as indicated. MR was detected using MR6F3A488 mAb. Images of each antigen labelling are shown separately in gray-scale, and merged in colour, in some cases with nuclear counterstaining shown in blue. Colocalisation of red and green is indicated by yellow. Examples of MHC class II-positive APC which coexppress MR are indicated with arrows. These constitute a subset of APC in the skin, liver, tongue and heart. No background was detected with an irrelevant IgG2aA488 negative control (shown in the spleen inset of MR-labeling only). Background labeling due to secondary Abs alone was also not detected in control sections (shown for anti-hamster IgG in the spleen inset of CD11c labeling only). Scale bars are indicated in MR-labeled panels only, and are 100μm.

From: Linehan SA. The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 2005 Feb 8;6:4.
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